Blastocyst Culture

Blastocyst culture

Until about ten years ago, it was impossible to consistently grow (or ‘culture’) embryos in a laboratory to the blastocyst stage. Specialized commercial culture media (the fluid in which embryos are produced in the lab) have now allowed embryos to be successfully nurtured to the blastocyst stage outside of the human body. This is known as ‘blastocyst culture’ in the laboratory.

After the egg retrieval procedure, fertilization is performed in the IVF laboratory using IVF (In-vitro fertilization) or ICSI (intra-cytoplasmic sperm injection), and the resulting embryos are placed in an incubator for the further development. The developmental stages are as follows:

  • The day of egg retrieval is referred to as Day 0. (IVF or ICSI is done after egg retrieval)
  • On the 1 day, the pronuclear stage (embryologist checks as to how many eggs have successfully fertilized)
  • On Day 2, there are two to four cell stages.
  • On day 3, there are eight cells.
  • On day 4, the morula stage
  • On day 5 or 6, the blastocyst stage

Historically, embryo transfer after IVF or ICSI was done on Day 2 or Day 3 after egg collection. However, developments in IVF laboratory technology now allow us to culture embryos for five or six days. This gives the embryos additional chances to demonstrate their developmental potential. Some embryos cease to develop (arrest) before day 5/6. This ‘natural selection’ enables the embryologist to discover and pick the best embryo/embryos for transfer.

Advantages of Blastocyst Culture

Blastocyst culture requires optimal laboratory conditions. Approximately 45% of fertilized eggs reach the blastocyst stage.

Because only the most competent embryos mature to the blastocyst stage, allowing embryos to grow and develop in the laboratory until they reach the blastocyst stage allows for the selection of the most capable embryo/s for embryo transfer.

In addition, in a natural pregnancy, the embryo takes around five days after fertilization to enter the womb, where it subsequently implants. On this day, the uterine lining is very receptive. As a result, blastocyst transfer is more physiological.

Blastocyst culturing is recommended for:

  • For patients with multiple failed IVF: Performing a blastocyst culture on a patient with a history of failed IVF with day2/3 embryo transfer, can yield more information related to the growth of the embryos and aid in improving pregnancy rate
  • To eliminate multiple pregnancies: Blastocyst culture allows the possibility of transferring the single best embryo and thereby reducing the chance of multiple pregnancy.

Contact with SaplingIVF clinic

SaplingIVF Clinic’s friendly and professional team is happy to answer any additional questions you may have regarding whether blastocyst culture and transfer is the right option for you.

What Our Patients Say

Khushboo Kumari
Khushboo Kumari
2023-01-23
Dr.Minal mam literally a very nice nd understanding. She always guide us nd encourage for positive thing..l'm so grateful to mam nd her staff...literally, I have no word to thanks mam nd her whole staff🥰🥰Thanku so much mam u r angel for me nd mine hubby🙂🙂
Dr.Mohd. Tarique
Dr.Mohd. Tarique
2023-01-21
Dr.Minal Mam is an amazing doctor as well as a wonderful person,from the bottom of my heart,,I want to say thank you but thank you is not enough to capture my gratitude towards her beautiful work☺️🙏well gratefullness thank you so much to start an auspicious journey of parenthood….. Your encouragement to help me get to the best time in my life
Vijay Hooda
Vijay Hooda
2023-01-17
Hum log shadi k 8 saal se try kar rahe the hume kabhi conceive nhi hua uske baad hum ne kayi dr ko dikhaya lekin dr Minal mam k paas aakr humko ek baar mein result mila aaj 2 month complete ho gaye hai mai or meri family bhut khush hai hum dr Minal mam and staff ko bhut shukriya krte hai or sub log bhut halpfull hai thanku sbhi ko jinko bhi bche hone mein dikkat hai go yha jrur aaye dr bhut ache h or staff bhi thanku sapling team
Anil Kumar
Anil Kumar
2023-01-16
Hi gyes! Meri shadi ko 4 saal ho gye the par bache ki khushi se mai vanchit thi...Maine sab jagha apna illaz karwaya bohat medicine Li...logo ne jo jo bola Kiya ..bohat si jagha hospital bhi gyi ...par kahi se bhi umeed Puri nahi hui...last mai ya bole to haar k mai sapling IVF center pe gyi jha sirf 3 mahine k treatment se mujhe vo khushi mili jisko Mai lafjo mai vya nahi kar sakti....mai maa ban saki....yaha such mai Mera sapna pura hua....mujhe pata hai maa Banna kisi aurat ko kitni khushi deta to mai khud k experience se un sab logo ko recommend karugi jo bacho ka sapna pura karne k liye taras rahe hai .....such mai yaha sabka sapna jarur pura hoga....yaha ka staff bhi itna acha hai bilkul family ki tarha treat karta....har ek staff itna khayal rakhta jaise vo Hamra apna ho👍🙂
Dimple Kaushik
Dimple Kaushik
2023-01-15
We were trying for a baby from the past 3 year and we feel very lucky to come in contact with Dr. Minal. We got positive results...She and her staff are wonderful, caring and kind... They treat their patients like a family members... Thank you 💖
Sparshana
Sparshana
2023-01-14
Services are very nice. All staff are very cooperative.
Sangeeta Devi
Sangeeta Devi
2022-12-31
Hum logo ko sapling mein aakr acha result Mila ... Hum khush hai yha aakr Dr Minal mam ne bhut acha treatment Kia sapling or Dr Minal mam ko bhut thankuu
Shuchi Goel
Shuchi Goel
2022-12-26
I do not have enough words to express my gratitude towards Dr. Minal and her whole team. We went to sapling only in Jan 2022 with a lot of self doubt and next to zero hope towards our journey of parenthood as one of the doctors earlier had told us that our only way ahead is IVF and that too only if we are lucky enough but Dr. Minal went through the reports and showed confidence that we don’t really need such an extensive invasive process of IVF and things are possible otherwise for us. Her team was so supportive from the nurses to even the support staff who made us believe in us and within two months we had a positive pregnancy test in our hands much to our disbelief without u der going IVF. If you are someone struggling with fertility issues and are unsure of what to do Sapling should be your first choice. They do not encourage unnecessary treatment for the sake of higher income but are always honest about the process, success rates and the options that are available to you. Today I am a proud parent and it would not have been possible without Dr. Minal’s medical guidance, empathy and care.
Akhilesh Kumar
Akhilesh Kumar
2022-12-19
Great work
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